萝卜叶绿体ATP酶β亚基的cDNA克隆及序列特征

cDNA cloning, sequencing and characterization of radish chloroplast ATPase beta subunit

在植物叶绿体 ATP酶复合体中 ,β亚基作为催化亚基具有极重要的地位。尽管一些植物叶绿体 ATP酶β亚基的基因和蛋白序列已被测定 ,但萝卜中尚未见相关报道。为了填补这一空白 ,采用 3′- RACE- PCR和 RT- PCR相结合的方法 ,首次克隆到了萝卜叶绿体 ATP酶 β亚基的含完整编码区及 3′- U TR的 c DNA,它全长 196 1bp,3′-端非编码区长 46 4bp,拥有一个 1497bp的开放阅读框架 ,共编码498个氨基酸。其氨基酸序列与已报道的其它植物中该亚基的序列高度同源 ,这种极高的同源性反映了叶绿体

The beta subunit is the catalytic one of plant chloroplast ATPase. Genes and proteins of the chloroplast ATPase beta subunit have been isolated and sequenced for some plants. However, there are no reports for the genes or proteins in radish yet. This paper presents clone results for the chloroplast ATPase beta subunit cDNA from radish using 3′ RACE PCR and RT PCR. The cDNA was 1961bp in length with a 464bp 3′ UTR and an ORF of 1 497bp encoding 498 amino acids. This protein showed high similarity with other known plant chloroplast ATPase beta subunits, verifing the importance of chloroplast ATPase in the plant photosynthesis process.