蛋白合成抑制剂促进TNFα诱导大肠癌细胞凋亡

Promotion of TNFα -induced apoptosis with cycloheximide in colorectal carcinoma cell line LoVo

目的探索放线菌酮和ＴＮＦ。协同诱导人大肠癌Ｌ。ＶＯ细胞凋亡的发生规律。方法用细胞体外培养方法，以放线菌酮和均小剂量作用于人大肠癌ＬｏＶｏ细胞，经Ｈｏｅｃｈｓｔ３３２５８荧光染色，光镜观察形态改变，并用流式细胞术检测ＤＮＡ断裂情况。结果单用ＴＮＦａ５万［Ｊ／Ｌ或放线菌酮４ｍｇ／Ｌ，均未引起ＬｏＶｏ细胞凋亡效应。而两者协同，作用１２ｈ至４８ｈ，ＬＯＶＯ细胞逐渐出现明显的凋亡形态学改变和ＤＮＡ断裂。结论小剂量的放线菌阴和ＴＮＦＱ协同作用于人大肠癌Ｌ。ＶＯ细胞，出现明显的凋亡效应。

Objective To explore the regulation of apoptosis in human colorectal carcinoma cell line LoVo induced by cooperationcycloheximide (CHX) with tumor necrosis factor- α (TNF α ). Methods LoVo cells were cultured in vitro and treated with smalldoses of CHX and TNF α. The morphological changes associated with apoptosis were observed under optical microscope afterHoechst 33258 staining, and DNA fragments were assayed by flow cytometry. Results LoVo cells did not undergo apoptosis shenthey were treated by only 4 mg/L CHX or 5 × 105 U/L.TNF α from 12 to 48 h, but did shell by combination CHX and TNt α. Thenlorphological changes associated with apoptosis were noted and DNA fragments detected. Conclusion Combinatioll of the smalldoses of COX and TNF α could induce obvious apoptosis effect on LoVo cell line.