三种方法测定血浆纤维蛋白原的方法学评价

METHODOLOGICAL APPRAISAL ABOUT THREE METHODS OFFIBRINOGEN DETERMINATION IN PLASMA

作者对血浆纤维蛋白原测定的三种方法进行了方法学评价和对照试验，结果表明：双缩脲法各项指标均较好，可做为参考方法；考马斯亮蓝微量比色法呈色稳定，线性好，准确度较高，与双缩脲法比较Ｙ＝０．９４８ｘ＋０．０８２，ｒ＝０．９７８４，Ｓ＿（ｙｘ）＝０．５８ｇ·Ｌ￣－，此法可作为临床常规测定血浆纤维蛋白原较好的方法；相比之下热沉淀法各项指标均不如以上两法。

We did methodological evaluation and comparison of three methods for fibrinogen determination in plasma. The results of our experiments prove that Biuret Method is better in every quality index tested and can be used as a reference method. For CBG Method,the linearity. accuracy and stability of it are satisfatory. Comparing CBG method with Biuret method,we establish the following regressive equation Y=0.945x+0.082(r=0. 9784,Sy·x=0. 58g·L(-1)). There fore,CBG Method can be used as a better routine method for fibrinogen determination in plasma in clinical loboratories.Heat-precipitation Method is worse than Biuret Method and CBG Method in every quality index tested.