用原位核酸杂交法探讨柯萨奇病毒与克山病的关系

Investigation on the relationship between Coxsackie virus infection and keshan disease with the nucleic acid hybridization in situ

为进一步探讨柯萨奇病毒与克山病的关系，采用柯萨奇Ｂ３病毒（ＣＶＢ３）制备的ｃＤＮＡ探针与黑龙江、山东、云南三省病区克山病尸检心肌组织及胎儿克山病心肌组织的石蜡切片进行了原位核酸杂交，并用脑外伤、ＣＯ中毒等意外死亡健康成人及冠心病、风心病尸检心肌组织和病区、非病区７～８个月引产胎儿心肌组织的石蜡包埋切片作为对照。结果急型克山病８／１３、慢型克山病３４／５０、亚急型克山病２２／２９呈现阳性杂交信号，提示有柯萨奇病毒ＲＮＡ的存在，冠心病２／１５、风心病１／１５、显著低于克山病，而健康成人对照组均为阴性，胎儿克山病一例呈阳性反应，病区引产胎儿心肌组织１０例中有２例也检出柯萨奇病毒ＲＮＡ，而非病区胎心则为阴性。结果提示在一些克山病的发生和发展过程与柯萨奇Ｂ组病毒的感染高度相关。

 In order to research the relationship between Coxsackie virus and Keshan disease,we labeled the CVB3 cDNA probe with bio11dVTP by nick translation and performed nucleic acid hybridization to examine paraffinembedded keshan disease myocardial specimens and fetus myocardial speciments from Heilongjiang,Shandong,and Yunnan provinces.Control myocardial samples included patients with cerebral trauma,carbon monoxide poisoning.Coronary heart disease,rheumatic heart disease and fetus aged 7￣8 months from nonendemic areas.The results showed that the positive findings were 8/13 in acute keshan disease,34/50 in chronic keshan disease,and 22/29 in subacrte keshan disease.But control myocardial samples were negative.The results suggested that the development of some keshan disease mignt be highly related to infection of Coxsackie virus.