三角褐指藻的培养条件研究

Study on Cultivation Conditions of Phaeodactylum tricornutum

对三角褐指藻 (Phaeodactylumtricornutum)进行了培养条件研究 ,结果表明 ,在 2 0℃下 ,采用去除氮源的No .4基本培养液 ,起始 pH值为 8.0 ,(NH2 ) 2 CO为氮源 ,氮浓度为 0 .0 1mol/L时 ,对藻细胞的光合自养生长极为有利 ,培养 10d ,其生物量达 4 4 1mg/L .过高的碳酸氢钠浓度 (>0 .0 4mol/L)易引起培养液pH值的过大改变而导致培养液中出现沉淀 ,在培养液中加入葡萄糖有利于藻细胞的生长 ,光合自养生长生物量浓度较低 ,混合营养明显促进藻细胞的生长 ,且培养液中同时含质量分数 0 .2 %葡萄糖和 0 .0 4mol/L碳酸氢钠后藻生物量达 16 30mg/L ,因此光合异养生长明显促进藻细胞的生长 .

The cultivation conditions of Phaeodactylum tricornutum , one of ocean microalgae, has been investigated in this paper. It indicates that biomass concentration was 441 mg/L in ten days cultivation period under the following photoautotrophic cultivation conditions: a culture at 20 ℃, the original pH 8.0, No.4 medium, urea as nitrogen source, nitrogen concentration 0.01 mol/L. The results show that photoautotrophic growth gives the lower biomass concentration than photoheterotrophy on sugars and P. tricornutum is a good source of EPA. This paper provides a good foundation for the further study of EPA potential production of P. tricornutum.