摘要
作为一种应激 ,运动会引起机体血液供应的重新分配。内皮素 (ET)和一氧化氮 (NO)是一对调节局部组织血液供应的拮抗因素。本实验采用递增强度的跑台运动方式 ,经过 7周训练 ,塑造了运动性疲劳大鼠模型 ;并通过原位杂交的方法 ,结合图像分析 ,观察了运动训练中不同负荷和运动后不同恢复时相的大鼠脑组织一氧化氮合酶 (NOS)和ET - 1mRNA的表达 ,以研究运动时脑组织中NOS和ETmRNA表达的动态变化规律 ,并探讨脑组织的血液供应与运动性中枢疲劳之间的关系以及纯中药制剂“体复康”的调节作用。研究结果表明 ,NOSmRNA在安静对照组大鼠脑组织海马和丘脑等部位均有基础表达 ;进行适量运动的训练对照组与安静对照组无明显差异 ,说明适量运动对大鼠脑组织中NOS活性影响不大 ;强化训练组表达明显减弱 ,说明大负荷运动会使脑组织中NOS活性减弱 ;而同样负荷的强化训练中药组表达明显强于强化训练组 ,说明该中药制剂可提高NOS活性 ;另外 ,在一次大强度的运动后即刻 ,强化训练组NOSmRNA表达明显减弱 ,至运动后 30分钟时略有恢复 ,到运动后 3小时时恢复到接近安静对照组的水平 ;而强训中药组在运动后即刻表达明显高于同时相的强化训练组 ,至运动后 30分钟时与强化训练组相同 ,到运动后 3小时时恢复到接近安静对照组的水?
Exercise, as a stress, can regulate the circulating blood volume. Both ET-1 and NO are the EDRF (endothelium derived relaxing factors), and are antagonistic to regulate the vasomoricity in topical tissue. In this paper, in situ hybridization (ISH) integrated image pattern analysis was used to explore the effect of “TiFuKang” on the gene expression of ET-1 and cNOS in cerebrum in exercise-induced fatigue rats with long term (total 7 weeks ) and high intensity (speed from 15m/min gradually increased to 35 m/min, 20 to 30 min/d duration)exercise. The main results were as follows: There were more gene expression of ET-1 and less gene expression of cNOS mRNA in hippocampus and thalamus in model group than that in control group, drill group and treatment group immediately after exercise, It suggested that cerebral vasospasm could be caused by the heavy exercise or mental and physical stress during exercise, and then led to the exercise-induced central fatigue due to the cerebral ischemia. It also suggested that the Chinese medicine, “TiFuKang” could significantly strengthen cNOS gene expression and obviously inhibited ET-1 gene expression in hippocampus and thalamus in rats with chronic exercise, and thus increase the cerebral blood flow (CBF)to prevent the exercise-induced central fatigue.
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2001年第2期116-120,共5页
Chinese Journal of Sports Medicine
基金
国家自然科学基金!委员会重点资助 NO :39430 140
