多聚酶链反应技术在麻风诊断中的应用研究

Study on the Applicatin of PCR on the Leprose Diagnosis

目的 了解用 16SrRNA作引物 ,用PCR方法 ,检测诊断麻风的可行性。方法 用麻风分枝杆菌 16SrRNA基因片段作引物 ,采用PCR技术 ,对 2 0余种非麻风分枝杆菌进行检测 ;同时也对麻风流行地区的 72名经传统方法诊断的麻风病人和 45名健康自愿受试者进行检测。结果 2者的检测结果进行比较 ;χ2 检验P >0 0 5 ,差异无显著性。进行了 2种方法检查结果的比较研究 ,结果也完全一致。结论 16srRNA作引物 ,用PCR检测诊断麻风病人与传统方法诊断结果基本一致。

Objective To understand the possibility of detecting the M.leprae by polymerase chain reaction (PCR) with 16SrRNA primer. Methods By PCR with 16SrRNA primer, more than 20 strains different mycobacteria and 72 leprosy cases diagnosed by traditional methods were tested. Results Both method showed almost same results.45 health volunteers in leprosy endemic area were also tested by both PCR and traditional methods. The result was same.Conclusion PCR detecting leprosy with 16SrRNA sequence as primer has almost same results compared with the traditional method for diagnosing leprosy patients.