丹参注射液对庆大霉素耳中毒豚鼠耳蜗NOS活性的影响

The Effect of Salvia Miltiorrhiza on Gentamicin Ototoxicity-Induced Activity of Nitric Oxide Synthase in Cochlea of Guinea Pig

目的 :探讨丹参注射液 (SalviaMiltiorrhiza ,SM)对庆大霉素 (gentamicin ,GM)耳毒性损伤的保护作用。方法 :制备豚鼠药物中毒性耳聋模型 ,应用NADPH 黄递酶 (NADPH diaphorase,NADPH d)组织化学染色及图像分析技术 ,并结合听性脑干反应 (auditorybrainstemresponse ,ABR)测试 ,观察SM对GM耳中毒豚鼠耳蜗一氧化氮合酶 (ni tricoxidesynthase ,NOS)活性的影响及其与听阈的关系。结果 :SM +GM组耳蜗NOS活性和ABR阈值均明显低于GM组 (P <0 .0 1) ;且ABR阈值与NOS活性变化高度相关 (rGM=- 0 .82 36～ - 0 .86 6 2 ;rSM +GM=- 0 .86 2 8～- 0 .9172 ,P <0 .0 1)。结论 :SM可通过降低耳蜗NOS活性以减轻GM的耳毒性损伤 ,从而改善听功能。

Objective: Our purpose was to explore the protective role of Salvia Miltiorrhiza (SM) on gentamicin (GM) ototoxicity. Methods: We used NADPH diaphorase in histochemical staining and image quantitative analysis technique, combined with auditory brainstem response (ABR) measurement to investigate the change of nitric oxide synthase (NOS) activity in cochlea of guinea pig after the injection of GM and SM. Results: Gebtamicin and SM significantly reduced NOS activity in cochlear and the threshold of ABR compared with GM alone (P<0.01); and the threshold of ABR was in high correlation with NOS activity (r GM = -0.8236 ～ -0.8662; r SM+GM = -0.8628 ～ -0.9172, P<0.01). Conclusion: Salvia Miltiorrhiza can reduce NOS activity in cochlea, alleviate GM ototoxicity, and ameliorate hearing function.