异丙酚对红细胞过氧化损伤的保护作用

Protective Effect of Propofol on the Peroxidative Injured Erythrocyte

目的 :观察临床相关浓度异丙酚对红细胞过氧化损伤的保护作用。方法 :采 2 0例健康成人静脉血制成RBC悬液 ,分为空白对照组、过氧化氢 (H2 O2 ,10 0mmol/L)损伤组及 3个损伤加不同浓度 (分别为 2 5 ,5 0及 75 μmol/L)异丙酚组 ,孵育后测定RBC悬液中K+、丙二醛 (MDA)浓度和RBC溶血度。结果 :孵育 6 0min ,加异丙酚组K+浓度 (0 .16 ,0 .14,0 .14mmol/L)、MDA浓度 (5 .6 6 ,5 .5 7,6 .2 0nmol/L)和RBC溶血度 (76 .89% ,5 9.84% ,6 4.2 2 % )与单纯损伤组 (分别为 0 .2 6mmol/L ,9.19nmol/L和 10 0 % )相比均显著降低 ,加异丙酚各组间及与空白对照组 (0 .10mmol/L ,4.13nmol/L ,5 2 .73 % )间无显著差别。结论 :临床相关浓度的异丙酚对H2 O2 损伤RBC可抑制MDA生成 ,减少K+外流和降低溶血度 ,具有抗RBC过氧化损伤作用 ,但浓度依赖性不明显。

Objective: Our aim was to observe the protective effect of propofol in clinical relevant concentration on the peroxidative injured erythrocyte. Methods: Intravenous blood samples taken from 20 healthy adults were prepared for red blood cell (RBC) suspensions and divided equally into 5 groups: groupⅠfor control, group Ⅱ with hydrogen peroxide (H 2O 2, 100 mmol/L) induced injury, and group Ⅲ, Ⅳ, Ⅴ with the same injury as the group Ⅱ but being pretreated with 3 different concentrations of propofol (25, 50, 75 μmol/L), respectively. The concentrations of potassium and malondialdehyde (MDA) in RBC suspensions and hemolytic degree after incubation were measured. Results: After 60 minute incubation, the extracellular potassium concentrations (0.16, 0.14, 0.14 mmol/L), MDA concentrations (5.66, 5.57, 6.20 nmol/L), and hemolytic degree (76.89%, 59.84%, 64.22%) decreased significantly in the groups that were pretreated with propofol as compared with the group Ⅱ (0.26 mmol/L, 9.19 nmol/L, and 100%), but no difference has been seen within the groups pretreated with 3 different concentrations of propofol and between the propofol treated groups and the group Ⅰ(0.10 mmol/L, 4.13 nmol/L, 52.73%). Conclusion: Propofol in clinical relevant concentrations may decrease MDA production, hemolytic degree, and potassium exflux from erythrocyte in response to in vitro oxidative challenge with hydrogen peroxide and enhance erythrocyte antioxidant capacity. The protective effect is not related with concentrations.