肾病综合征患儿巨细胞病毒感染的初步研究

Human Cytomegalovirus Infection in Children with the Nephrotic Syndrome

目的 探讨肾病综合征 (NS)与人巨细胞病毒活动性感染的关系。方法 用聚合酶链反应 (PCR)技术和间接ELISA法检测 36例NS患儿外周血白细胞中的HCMV DNA及血浆中的HCMV IgM。 结果 36例NS活动期患儿HCMV IgM阳性 1 1例 ,阳性率30 .5 % ,HCMV DNA阳性 1 4例 ,阳性率 38.9% ,与对照组阳性率(1 2 .0 % ,4.0 % )比较差异有显著性 (P <0 .0 1 ) ;HCMV IgM和HCMV DNA均为阳性的有 8例 ,阳性率为2 2 .2 % ,与对照组 (4% )比较差异有统计学意义 (P <0 .0 1 )。结论 部分NS活动期患儿存在HCMV感染。

Objective To study the relationship between the nephrotic syndrome(NS) and active infection with human cytomegalovirus (HCMV). Methods HCMV DNA in peripheral leukocytes from 36 cases of NS was detected with the polymerase chain reaction (PCR) technique. HCMV IgM antibody detection was done using ELISA simultaneously. Results The positive rates of active HCMV IgM antibody and HCMV DNA were 30.5% and 38.9% , respectively, higher than those of the control group (both P< 0.01 ). HCMV IgM and HCMV DNA were found simultaneously positive in 8 cases. Conclusions Some children with NS have HCMV infection.