小鼠免疫力对接种人类肝癌细胞系9724生长的影响

Immune affects growth of inoculated human hepatocarcinoma cell strain 9724 in mice

目的 探讨不同免疫力小鼠的免疫细胞对人类肝癌细胞系 972 4生长的影响及异种特异性细胞毒杀伤能力 .方法 将培养后的人类肝癌细胞系接种到不同免疫力小鼠 (免疫正常的 BAL B/ c,B缺陷的 CBA/ N,T缺陷的 BAL B/ c nude及 T,B缺陷的 C.B- 17SICD小鼠 )右后臀皮下 ,观察其生长 ;取实验和相应的正常鼠脾细胞进行细胞毒杀伤试验 .结果 BAL B/ c和 CBA/ N小鼠不成瘤 ;BAL B/ c nude和 C.B- 17SCID小鼠 10 0 %成瘤 ;SCID免疫重建组用 BAL B/ c外周淋巴细胞重建 SCID(BAL B/ c- PBL- SCID)的不成瘤 ,用 CBA/ N外周淋巴细胞重建的 SCID(CBA/ N- PBL - SCID)成瘤 ,接种过瘤的 BAL B/ c和 CBA/ N小鼠脾细胞对癌细胞有较强的细胞毒杀伤作用 ,对照组和 nude,SCID小鼠无杀伤作用 ;SCID免疫重建有较小的杀伤作用 .结论 T细胞在异种瘤移植排斥中起主要的免疫杀伤作用 ,这种排斥是异种特异性的 ;肿瘤免疫需要综合性免疫作用 .

AIM To explore the effect of immune cells in various mice with different immune on growth of human hepatocarcinoma cells in these mice and xenospecific cytotoxicity of these mice to hepatocarcinoma cells. METHODS The human hepatocarcinoma cells cultured were inoculated subcutaneous into the posterior limbs in various mice with different immune (including normal immune mice-BALB/c, B lymphocyte deficient mice-CBA/N, T lymphocyte deficient mice-BALB/c nude and T, B lymphocytes combined deficient mice-C.B-17 SCID) and their growth in the mice. Were surveyed And the spleen cells were harvested from both experiment groups in 5 weeks after being inoculated and from control groups a to examine direct cytotoxicity to the hepatocarcinoma cells. RESULTS There were no carcinoma growth in BALB/c, CBA/N mice and immunoreconstructed SCID mice with BALB/c mice peripheral blood lymphocytes (BALB/c-PBL-SCID) but 100% carcinoma growth in SICD, nude and immunoreconstructed SCID mice with CBA/N mice peripheral blood lymphocytes (CBA/N-PBL- SCID). And the spleen cells in experiment groups of BALB/c and CBA/N mice displayed strong cytotoxicity to target cells and the lymphocytes of immunoreconstructed SCID mice generated a little lysis of target cells, but those of control groups and nude, SCID mice generated no lysis of target cells. CONCLUSION Tlymphocytes which are xenospecific cytotoxic mayplay a main role of immune killing in rejection to xenografted tumor but the comprehensive immune may be necessary in the tumor immunity.