HCCA1在原发性肝癌中的表达及其意义

Expression and clinopathological significance of HCCA1 in hepatocellular carcinoma

目的 对作者近期克隆的新基因HCCA1与肝癌的相关性进行研究。方法 采用Northern杂交分析HCCA1mRNA在肝癌中的表达 ,应用制备的特异性抗体检测其蛋白水平的表达 ,结合临床及病理学资料进行分析。结果 6 5例HCC患者在其癌组织中HCCA1mRNA表达的检出率为 90 8% (5 9/ 6 5 ) ,其中 91 5 % (5 4/ 5 9)患者HCCA1mRNA在肝癌组织中的表达水平明显高于癌旁组织 ,有 13 8% (9/ 6 5 )患者在癌旁组织中检测到较弱的HCCA1mRNA的表达 ,86 2 % (5 6 / 6 5 )患者在癌旁组织中未检测到HCCA1mRNA的表达。与病理指标的相关性分析表明 ,HCCA1mRNA在Ⅲ～Ⅳ级肝癌中的表达水平明显高于Ⅰ～Ⅱ级肝癌 ,有门脉癌栓形成者的表达水平明显高于无癌栓形成者。免疫组织化学在蛋白水平上证实HCCA1在肝癌中的高表达。结论 HCCA1是一个新的肝癌相关基因 ,可能在肝癌的浸润和转移中起重要作用。

Objective To explore the correlation of a newly cloned HCCA1 gene expression with hepatocellular carcinoma (HCC). Methods HCCA1 mRNA and protein expression were detected by Northern blotting and by immunohistochemistry respectively. Its clinopathological significance was also analyzed. Results HCCA1 mRNA expression was found in 59 (90.8 %) out of 65 patients. Of these, 54 (91.5 %) showed significantly elevated expression of HCCA1 mRNA in HCC tissues. There were 9 (13.8%) patients who had low level of HCCA1 mRNA expression in paracarcinoma, and 56 (86.2%) cases showed no detectable signals. HCCA1 mRNA expression was closely related to lower tumor grading and portal vein invasion. It was noted that HCCA1 mRNA levels were higher in tumor grading Ⅲ Ⅳ group than in grading I Ⅱ group, higher in portal vein thrombus group than in non thrombus group. Immunohistochemical staining confirmed that the HCCA1 protein was preferentially expressed in HCC tissues. Amongst 38 patients examined, 20 (52.6 %) revealed positive staining with anti HCCA1. Conclusion HCCA1 is a novel HCC associated gene which may play a role in HCC invasion and metastasis.