银杏叶提取物对沙土鼠脑缺血再灌注损伤的影响

Protective effects of Ginkgo biloba extract on cerebral ischemia-reperfusion injury in gerbils

目的 :研究银杏叶提取物对沙土鼠脑缺血再灌注损伤的影响。方法 :采用夹闭沙土鼠双侧颈总动脉 10min或 2 0min再灌注 5d或 1d ,造成沙土鼠前脑缺血再灌注损伤模型。 95只动物分为假手术组、缺血再灌注组、银杏叶提取物 (GbE) 50mg·kg 1组及GbE10 0mg·kg 1组。于缺血前 2d和再灌注期间ig给药 ,观察GbE对脑组织钙、钠、水含量和脂质过氧化物的影响 ,以及对海马CA1区神经元迟发性死亡的保护作用。结果 :GbE能降低沙土鼠缺血再灌注后大脑皮层含水量 ,减轻钙、钠积累 ,且具有剂量效应相关性 ;GbE10 0mg·kg 1能降低缺血再灌注 1d内动物的死亡数及脑组织丙二醛含量 ,增加脑缺血后海马CA1区神经元密度。结论 :GbE对脑缺血再灌注损伤有保护作用。

AIM: To study the effects of Ginkgo biloba extract ( Gb E) on cerebral ischemia reperfusion injury. METHODS: Cerebral ischemia reperfusion injury was produced by 10 min or 20 min occlusion of bilateral carotid arteries followed by 5 d or 1 d reperfusion in gerbils. Ninety five gerbils were divided into 4 groups: sham operation, ischemia reperfusion, Gb E 50 mg·kg 1 and Gb E 100 mg·kg 1 groups. Drugs were given intragastrically 2 d prior to ischemia and during reperfusion. The effects of Gb E on the contents of calcium, sodium, water in cortex, and lipid peroxide(LPO) in brain hemispheres, as well as the density of neuron in hippocampal CA 1 sector were observed. RESULTS: Gb E could reduce the increase of calcium, sodium, water content in a manner of dose depedance. The dosage of Gb E 100 mg·kg 1 could decrease the content of LPO and the mortality, increase the density of neuron in hippocampal CA 1 sector. CONCLUSION: Gb E has protective effects on cerebral ischemia reperfusion injury.