乳猪脑活性多肽的制备,成分分析及实验动物抗脑老化研究

Preparation,Component Analysis and Anti-Ageing Effect of Brain Active Peptide of Sucking Pig Brain

目的:制备乳猪脑活性多肽替代价格昂贵的进口脑活素,用于临床治疗。方法:乳猪脑组织经匀浆、脱脂、加热、消化、离心、超滤,制备脑活性多肽,并与奥地利产脑活素对比进行理化检测,并用SAM-P/8早老鼠进行脑活性多肽抗脑老化的研究。结果:高效液相色谱分析,乳猪脑活性多肽峰型分布与奥地利产脑活素基本一致。氨基酸成分分析,一些氨基酸含量高于奥地利产脑活素。结论:制备的脑活性多肽注射液符合中华人民共和国卫生部《WS-198(X-171)-97(1)》有关脑蛋白水解物注射液部标准(试行)。

Objective:To study the preparation,component and anti-ageing effect of the brain active peptide of sucking pig brain. Methods:Through homogenate, degrease, heat, hydrolyze, centrifugation and ultrafiltration of sucking pig brain tissue, the brain active peptide was prepared. It was measured by physical and biochemical methods and its anti-ageing effect was co-mared compared with the expensive Cerebrolysin made in Austria. Results: The analysis of high pressure lipid chromato-graphy(HPLC) showed that both peak areas of the brain active peptide and Cerebrolysin were identical and some amino-acid contents of the brain active peptide were more than those of Cerebrolysin. Conclusion:The product of brain active peptide ac-cords with the PRC Hygiene Ministry standard about brain protein hydrolytic reagent injection 《WS-198(X-171)-97(1)》.