大鼠脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T_3受体关系

RELATIONSHIP BETWEEN SPLEEN-ASTHENIA SYNDROME AND SERUM THYROXIN,T_3 RECEPTORS OF HYPOTHALAMUS CELLS & THYMUS T LYMPHOCYTE NUCLEI IN RATS

目的 :探讨脾虚证与血清甲状腺激素及下丘脑、胸腺细胞核T3受体的关系。方法 :成年SD大鼠 32只随机分为 4组 (每组 8只 ) :A组 (正常组 )、B组 (脾虚组 )、C组 (治疗组 )、D组 (自复组 )。采用放射配基分析法 ,测定各组大鼠血清T3、T4 、FT3、FT4 、γ T3及下丘脑、胸腺细胞核T3 受体的含量。结果 :B、D组大鼠血清T3、T4 、FT3、FT4 含量均较A、C组低 ,有显著差异 (P <0 .0 5或P <0 .0 1) ;各组间血清γ T3含量无显著性差异 ;B、D组下丘脑、胸腺细胞核T3 受体含量较A、C组低 ,有显著差异P <0 .0 1)。结论 :血清甲状腺激素及下丘脑、胸腺细胞核T3 受体的水平降低 ,可导致甲状腺激素生物效应低下 ,经由神经内分泌免疫调节环路 ,削弱免疫功能 。

Objective:To study the relationship between Spleen asthenia syndrome and serum thyroxin,T 3 receptors of hypothalamus cells & thymus T lymphocyte nuclei in rats.Method:Rhubarb decoction was used to develop spleen asthenia syndrome in SD adult rat.32 rats were averagely divided into four groups at random:Group A(control group),group B(spleen asthenia group),group C(group treated with Sijunzi decoction),group D(spontaneous rehabilitation group).The radioimmunoassay was applied to measure serum thyroxin levels and the contents of T 3 receptors of hypothalamus cells and thymus T lymphocyte nuclei of every rat.Result:The serum levels of T 3,T 4,FT 3,FT 4,the contents of T 3 receptors of hypothalamus cells and thymus T lymphocyte nuclei in group B,D were lower than those in group A,C.The difference between them was significant.Serum level of γ T 3 in four groups had no significant difference.Conclusion:The decline of T 3,T 4,FT 3,FT 4 serum levels,and the contents of nuclear T 3 receptors in hypothalamus cells & thymus T lymphocytes can influence the activity of thyroxin,then the immunity function will be impaired via neuroendocrinimmunology net(NEI net),this may be a kind of important pathogensis of spleen asthenia syndrome.