鸡Ⅱ型胶原蛋白的药物动力学研究

PHARMACOKINETICS OF CHICKEN TYPE Ⅱ COLLAGEN IN RATS

目的 :研究了鸡Ⅱ型胶原 (chickentypeⅡcollagen ,CCⅡ )在大鼠体内的药物动力学。方法 :采用氯胺T法进行12 5I CCⅡ标记 ,放化纯度 >96 2 6 %。给大鼠灌胃 7 4× 10 5、1 48× 10 6及 2 96× 10 6Bq·kg-1的12 5I CCⅡ ,然后测定 9个时间点的血药浓度 ,用一室模型进行参数计算。大鼠灌胃 2 96× 10 6Bq·kg-1后 ,测定 1、3、6h的分布及排泄情况。结果 :12 5I CCⅡ的吸收相较短 ,t1/ 2Ka约为 0 5 6～ 0 74h。消除较快 ,t1/ 2Ke约为 3 6 0～ 4 32h。该药分布较快 ,以肾、胃、肠、肺、肝、脾的分布量较高。在 72h内从尿及粪中分别排出 2 7 9%和 4 8% ,2 6h内从胆汁中排出6 7%。结论 :CCⅡ口服给药后 ,吸收较快 ,消除也较迅速 。

OBJECTIVE:To study the pharmacokinetic characteristics of chicken type Ⅱ collagen (CC Ⅱ) in rats METHODS:To use Chloramines T method to label CCⅡ by 125 I, the radiochemical purity is more than 96 26% Administrated orally with three different doses (7 4×10 5、1 48×10 6、2 96×10 6 Bq·kg 1 ) of 125 I CCⅡ, 9 time points of blood concentrations were detected The pharmacokinetic parameters were calculated by 3p87 program and the radioactive drug plasma concentration fitted well to one compartment model The blood, urine, bile, feces and tissue sample are collected and assessed for excretion and distribution RESULTS:The results show that the absorbing time of CC Ⅱ is short ( t 1/2Ka is 0 56～0 74 h), the elimination are quick ( t 1/2Ke is 3 60～4 32 h) Tissue distribution in kidney, stomach, intestine, lung, liver and spleen are higher than others after 1 h Compared to the total doses administrated, 27 9% of the radian excreted in urine and 4 8% in feces in 72 h and 6 7% in bile with in 26 h CONCLUSION:After oral administration, CC Ⅱ is rapidly absorbed and it distributes broadly in the tissue