阿魏酸钠对大鼠脑微血管内皮跨膜电阻的影响

EFFECT OF FERULIC ACID SODIUM ON INCREASE OF THE PIAL MICROVASCULAR PERMEABILITY INDUCED BY HISTAMINE IN RAT

目的 :观察阿魏酸钠对组胺引起的脑软膜微血管通透性增加的抑制作用。方法 :脑软膜微血管通透性用跨脑软膜微血管内皮的电阻表示 ,跨脑软膜微血管内皮的电阻通过将微电极插入微血管腔内记录 ,并用电缆公式计算获得。结果 :脑软膜微血管经灌流人工脑脊液 ,测得的跨内皮电阻大约为 2 5 0 0Ω·cm2 ,说明微血管内皮是具有较低通透性的致密屏障 ,在人工脑脊液中加入 10 4 mol·L 1组胺引起跨内皮电阻快速的和可逆性的下降。在配对实验中 ,10 4 mol·L 1组胺引起跨内皮电阻的下降能够被在人工脑脊液中加入 10 5mol·L 1阿魏酸钠所抑制。结论 :结果表明组胺能够引起脑微血管通透性增加 。

OBJECTIVE:To study the effect of ferulic acid sodium on the increase of the pial microvascular permeability induced by histamine METHODS: The microvascular permeability was expressed by changes in the transendothelial electrical resistance which was measured with technique using microelectrode impaled into the vascular lumen and based on cable analysis of vessels in rat RESULTS:The transendothelial electrical resistance of microvessels superfused with artificial cerebrospinal fluid was about 2 500 Ω·cm 2 , indicating a tight barrier with extremely low ion permeability, and application of 10 4 mol·L 1 histamine in superfusate caused a rapid and reversible decrease of resistance In paired experiment, 10 4 mol·L 1 histamine induced decrease of transendothelial electrical resistance was inhibited by adding 10 5 mol·L 1 ferulic acid sodium in superfusate CONCLUSION:The results indicated that increases in the microvascular permeability can be induced by histamine, and ferulic acid sodium can inhibit the increases in the microvascular permeability induced by histamine It is necessary that the cellular mechanism of permeability response induced by ferulic acid sodium remains to be further elucidated