标本处理方法对聚合酶链反应检测结核分支杆菌结果的影响

Influence of specimen processing method on Mycobacterium tuberculosis detection by PCR-reversed dot hybridization

目的 探讨应用十二烷基硫酸钠 (SDS)处理标本在结核分支杆菌聚合酶链反应 (PCR)中的价值。方法 从 186例活动性结核病人收集痰、胸腹水、脑脊液、尿、血液等标本 2 6 6份 ,标本分别用SDS和常规方法进行处理。两种方法处理的标本同时用PCR 反相膜杂交试验检测结核分支杆菌 ,并将结果进行比较。结果 用SDS和常规方法处理的标本 ,PCR 反相膜杂交试验结核分支杆菌的检出率分别为 6 5 0 % (173/ 2 6 6 )和 5 1 5 % (136 / 2 6 6 )。通过配对计数资料卡方检验 ,SDS法和常规法之间的差异具有非常显著性意义 (χ2 =35 0 3,P <0 0 0 1)。结论 SDS法可以应用于临床标本结核分支杆菌PCR检测 ,提高结核分支杆菌PCR的检出率。

Objective To evaluate specimen processing with sodium dodecyl(lauryl) sulfate(SDS) on the detection of Mycobacterium tuberculosis by PCR reversed dot hybridization. Methods Totally 266 specimens were collected from sputum,pleural fluid,ascites,urine or blood of patients with active tuberculosis and were treated with SDS or routine method All specimens handled were tested for detecting M tuberculosis by PCR reversed dot hybridization. Results The detection rate of M tuberculosis from specimens prepared by SDS method was 65 0%(173/266) as compared with 51 5%(136/168) by routine method Results from two methods were considered statistically significant (χ 2=35 03, P <0 001) Conclusion The SDS processing method may be used to detect M tuberculosis from clinical specimens by PCR and enhances the detection rate of M tuberculosis