广枣总黄酮对阿霉素致大鼠心肌细胞过氧化损伤的保护作用

Protective Effect of Total Flavonoids of Fructus Choerospondiatis on Adriamycin-induced Rat Cardiac Peroxidation

目的:研究TFFC对大鼠心肌细胞过氧化损伤的保护作用。方法:将TFFC应用于ADR致过氧化损伤的大鼠,测定大鼠血清中LDH、AST、CK的活性,心肌组织中SOD、GSH-Px的活性以及MDA含量,并利用培养的乳鼠心肌细胞,观察培养液中LDH活性、MDA含量。结果:ADR组大鼠血清中LDH、AST、CK活性及心肌中MDA含量比正常对照组显著增高(P<0.05,P<0.001),而心肌中SOD、GSH-Px活性下降(P<0.001)。乳鼠心肌细胞加ADR培养组培养液中LDH活性、MDA含量显著高于正常对照组(P<0.001)。当TFFC干预后血清中LDH、AST、CK活性和MDA含量逐渐回落,SOD、GSH-Px活性上升;细胞培养液中LDH活性、MDA含量逐渐下降(P<0.01,P<0.001)。结论:TF-FC通过清除自由基,抑制脂质过氧化反应,维持心肌细胞膜结构和功能的完整性,而对心肌起保护作用。

Objective:To study the protection effect of total flavnoids of Fructus Choerospondiatis (TFFC) on rat cardiac muscle peroxida-tion in vitro and vivo. Methods: Adriamycin-induced peroxidation model on rat was adopted. Activities of LDH, AST and CK in rat' s serum, activities of SOD, GSH-Px and content of MDA in rat cardiac muscle were determinated. Activity of LDH and content of MDA in cultured suckling rat cardiac cell were also determinated. Results: Compared with normal group, activities of LDH, AST, CK in serum and content of MDA in car-diac muscle on adriamycin group increased (P< 0.05,P< 0.001) .activities of SOD,GSH-Px decreased in cardiac muscle (P< 0.001) . Activity of LDH and content of MDA in cultured fluid of suckling rat cardiac cell were higher than that of normal group (P< 0.001) .To various dosages TFFC groups,activity of LDH,AST,CK in serum and content of MDA in cardiac muscle decreased; activities of SOD and GSH-Px in cardiac muscle increased;activites of LDH and content of MDA in cultured fluid decreased (P<0.01,P<0.001). Conclusion:TFFC can maintain the integrity of cytomembrane by scavenging free radicals and inhibiting lipid peroxidation, namely, TFFC has an effect of protecting cardiac muscle.