TLC和HPLC法鉴别两种小粒菟丝子

Identification of Seeds of Cuscuta australis and C. chinensis by TLC and HPLC

采用TLC和HPLC两种方法鉴别菟丝子(Cuscuta.chinensis Lam.)和南方菟丝子(C.australis R.Br.)。TLC采用聚酰胺薄层层析,MeOH-HOAc-H_2O和CHCl_3-MeOH-HOAc两种溶剂二次展开。HPLC采用Hypersil-ODS柱,MeOH-0.025mol·L^(-1)H_3PO_4为流动相,流速1.0ml·min^(-1),检测波长360nm,柱温40℃。两种方法鉴别特征均很显著,且操作简便,重现性好。

Idenfitication of seeds of Cuscuta australis R.Br.and C.chinensis Lam.was carried out by TLC and HPLC.Polyamide membrane was used as stationary phase,MeOH-HOAc-H2O and CHCl3-MeOH-HOAc were used as mobile phase for TLC.For HPLC,Hypersil-ODS column was used; the mobile phase was MeOH-0.025 M H3PO4; the flow rate was 1.0 ml·min-t; detection wavelength was 360 nm; and column temperature was 40℃.Both methods represented significant identification characteristics,and were simple,accurate and reproducible.