后基因组计划中的蛋白组分析新方法——多重色谱与质谱

The novel methods for proteome analysis in post-genome project:multi-chromatography and mass spectrometry

双相聚苯稀酰氨凝胶电泳 (2 D PAGE)与质谱 (MS)是分辨和鉴定蛋白的最常用的方法 ,但也存在有缺点。本文介绍一些分离和鉴定蛋白质的新方法 ,包括用高效液相色谱 (HPL C)、毛细管等电聚焦 (CIEF)、毛细管电泳 (CE)或微毛细管色谱的 1D和 2

Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification.But it exist shortcomings.In this paper introduce some novel methods for protein resolution and identification,including the methods by high liquid chromotography,capillary isoelectric focusing,capillary electrophoresis or 1D and 2D microcapillary chromotagraphy.