^(60)Coγ射线全身照射后大鼠脑GAP-43 mRNA的表达增强

^(60)Coγirradiation enhances expression of GAP-43 mRNA in rat brain

目的 研究6 0 Coγ射线照射后大鼠脑GAP 43表达的变化与神经再生的关系。方法 地高辛标记的cRNA探针原位杂交。结果 正常对照大鼠除海马锥体细胞层有较强的阳性标记外 ,其他脑区仅有弥散的GAP 43mRNA弱阳性标记。 8Gy6 0 Coγ射线照射 1周后 ,大脑皮质、基底核、丘脑和下丘脑的大部分核团GAP 43呈中等强度的表达 ,照射后第 4周 ,以上脑区的GAP 43mRNA水平明显升高 ,第 7周GAP 43的表达逐渐减弱 ,但仍高于正常水平。结论 6 0 Coγ射线照射致大鼠脑损伤后GAP 43mRNA表达增强 。

Objective\ To study the relationship between the expression of GAP\|43 mRNA and nerve regeneration in rat brain after 60 Co γ\|irradiation. Methods\ Wistar rats were subjected to whole\|body irradiation with 8 Gy 60 Co γ\|rays.The expression of GAP\|43 was detected by in situ hybridization histochemistry using Dig\|cRNA probe. Results\ It was found that the expression of GAP\|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ\|irradiation.The peak of GAP\|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion\ Enhanced expression of GAP\|43 mRNA after 60 Co γ\|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.