低剂量照射诱导A549和2BS细胞适应性反应的研究

Study on adaptive responses of cells A549 and 2BS induced by low does irradiation

目的 观察A5 49细胞 (肺癌细胞 )和 2BS细胞 (人胚胎肺成纤维细胞 )低剂量照射后能否诱导适应性反应 ,探讨肿瘤细胞与正常细胞在诱导适应性反应方面存在的差异 ,并结合细胞周期变化 ,进一步阐明适应性反应形成机理。方法 1 应用克隆形成法和苔盼蓝细胞染色计数法 ,分别绘制了A5 49细胞和 2BS细胞的剂量 存活曲线。 2 应用流式细胞技术 ,对不同剂量照射后A5 49和2BS细胞周期进行了分析。结果 1.2BS细胞经低剂量照射诱导出适应性反应 (预先给予低剂量照射组的剂量 存活曲线与未预照射组比较 ,P <0 .0 1)。A5 49细胞经低剂量照射未诱导出适应性反应(P >0 0 5 )。2 .2BS细胞经低剂量加高剂量照射组于照后 30min即出现明显的G2 期阻滞 ,且细胞周期于 2 4h内恢复。结论 低剂量照射A5 49细胞未诱导出适应性反应 ,而 2BS细胞可诱导出适应性反应 。

Objective\ To investigate whether or not adaptive response of the 2BS and A549 cells induced by low dose irradiation.Moreover,to analyze the relationship between the adaptive response and cells cycle regulation. Methods\ 1.The dose survival curves of A549 and 2BS cells were figured by colony formation and typan blue cell staining.2.Flow cytometry was used to analyze the cell cycle after the different doses irradiation. Results\ 1. The adaptive response induced in 2BS cells by low dose,while it was not induced in A549 cells.2.G 2 phase arrest of 2BS cells after 75mGy X ray plus 4Gy 60 Co γ ray irradiation appeared in 30 minutes,and cell cycle recovered in 24 hours. Conclusion\ The adaptive response of 2BS cell could be induced by low dose irradiation,and there was close relationship between the adaptive responses and the regulation of cell cycle.