邻苯二酚类螯合剂对辐射引起大鼠肝线粒体损伤的保护作用

Protective effects of catecholomic acid derivatives on radiation induced damage of rat liver mitochondria

目的 观察邻苯二酚类螯合剂 95 0 1、95 0 2、76 0 1(CBMIDA)对辐射引起大鼠肝线粒体损伤的保护作用 ,以探讨其防护核素内污染辐射损伤的可能性。方法 采用γ射线外照射方法 ,引起大鼠肝线粒体辐射损伤 ,给予不同剂量的螯合剂 ,观察其保护作用。以硫代巴比妥酸比色法测定脂质过氧化产物MDA含量 ;在 5 2 0nm波长测定反应体系的浊度变化 ,以反映线粒体肿胀损伤程度 ,并制备电镜切片。结果 95 0 1(5× 10 - 6 mol L)、95 0 2 (10 - 5 mol L)、76 0 1(10 - 5 mol L)能显著抑制 12 0Gyγ辐射所致肝线粒体MDA生成 ,95 0 1、76 0 1对辐射引起的线粒体肿胀也有明显的保护作用 ,95 0 1并能防止辐射所致线粒体形态的病理性损伤。作者并对此类螯合剂对大鼠肝线粒体辐射损伤的保护作用机理进行了初步探讨。结论 邻苯二酚类螯合剂 95 0 1、95 0 2、76 0 1(CBMIDA)

Objective\ To evaluate the effects of catecholomic acid derivatives 9501,9502 and 7601 (CBMIDA) against radiation induced injury of rat liver mitochondria in vitro. Methods\ The injury of rat liver mitochondria was induced by γ irradiation in vitro. The contents of MDA were assayed by spectrophotometry of TBA.The absorption value at 520 nm was measured to detect swelling of mitochondria.The electron microscopic samples of mitochondria were prepared. Results\ All 9501 (5×10 -6 mol/L),9502(10 -5 mol/L),and 7601(10 -5 mol/L) significantly inhibited radiation induced increase of MDA information.The swelling of mitochondria induced by irradiation was also prevented by 9501,7601.The electron micrographs also showed that 9501 markedly reduced the pathological damage of mitochondria induced by γ irradiation.The mechanisms of antioxidative action of catecholomic acid derivatives was discussed. Conclusion\ Injurious effect of radiation on rat liver mitochondria can be prevented by catecholomic acid derivatives 9501,9502 and 7601 (CBMIDA).