CD34在放射损伤小鼠骨髓细胞的表达及其意义

Preliminary study on expression of CD34 in murine bone marrow cells with radiation injury and its significance

目的 研究辐射所致造血功能障碍时造血干 祖细胞表面CD34抗原表达的变化及其意义。方法 采用逆转录聚合酶链反应 (RT PCR)及斑点杂交的方法 ,初步检测了昆明种小鼠受 6 0Gy辐照后不同时相点时骨髓单个核细胞CD34表达量的变化 ,并对其可能的机理及其临床意义进行了探讨。结果 放射损伤后小鼠骨髓单个核细胞CD34表达在受照后 2 4h即明显增高 ,之后一直维持在较高水平 ,至照后 15d时仍较正常时高 5 0 %。结论 在此照射剂量时小鼠骨髓内残存的、具有造血功能的早期造血祖细胞在骨髓造血的内稳态遭到破坏后 ,可代偿性地迅速增加其增殖能力和细胞数量 ,以加速造血的恢复过程。

Objective\ To analyses the expressional change of the CD34 in bone marrow hematopoietic cells post\|irradiation. Methods\ Female Kunming strain mice irradiated with 6 Gy γ\|rays without any treatment were used to analyse the CD34 expression in bone marrow mononuclear cells at 24 h,day 3,5,7,10,12 and 15 after irradiation.Collected bone marrow mononuclear cells were used to extract the total RNA.Using the methods of RT\|PCR and dot blot hybridization with mouse CD34 specific primers and probe to measure the relative expression quantity. Results\ The CD34 expression was obviously increased as soon as 24 h post\|irradiation.Afterwards,it maintained at a higher level than normal until day 15. Conclusion\ The results indicate that after 6 Gy irradiation,the earlier hematopoietic progenitors can increase the irproliferative ability and the quantity compensatively,and would have important significance in hematopoietic repair post\|irradiation.