摘要
从南宁市郊土壤中筛选到的一株抗杀菌剂福美双的抗性菌T46,在福美双浓度达1500 μg/mL的GBM平板上仍能生长。经对T46的个体形态,培养特征的观察及生理生化试验,初步鉴定为假单胞菌属第一群铜绿假单胞菌。用广谱性考斯(cosmid)载体pLAFR1,在大肠杆菌ED8767中建立了T46的基因文库。转化子数和重组质粒酶切检查均证明,所构建的基因文库达到了理论要求。
A Pseudomonas strain T46 isolated from the suburban soil of Nanning was identified to be resistant to the fungicide Thiram.T46 can still grow well at a Thiram concentration of 1500 μg/mL.The characteristics in taxonomy and biochemical experiments showed that T46 was as a member of Pseudomonas aeruginosa.The gene bank of T46 was successfully constructed in Escherichia coli ED8767,using the broad host range cosmid vector pLAFR1.The constructed gene bank was proved to reach the theoretical standard.
