摘要
耐旱是水稻抗逆研究中最重要的性状之一。利用水稻籼粳品种窄叶青８号（ＺＹＱ ８）和京系 １７（ＪＸ１７）及其通过杂交Ｆ１代花药培养获得的由 １２７个单株组成的双单倍体分离群体（ｄｏｕｂｌｅ ｈｏｐｌｏｉｄ， ＤＨ）为材料，在营养液中培养 １０天后，对影响抗旱能力的根部几个主要性状进行了分析，发现最大根长（Ｍａｘｉｍｕｍ Ｒｏｏｔ Ｌｅｎｇｔｈ， ＭＲＬ）、根干重（Ｄｒｙ Ｒｏｏｔ Ｗｅｉｇｈｔ，ＤＲＷ）和根茎干重比（Ｒｏｏｔ／Ｓｈｏｏｔ Ｒａｔｉｏ ｏｆ Ｄｒｙ Ｗｅｉｇｈｔ， ＲＳＲ）３个性状在群体中变异较大。利用该群体建立的水稻分子遗传图谱，对上述 ３个水稻性状进行数量性状座位（ＱｕａｎｔｉｔａｔｉｖｅＴｒａｉｔ Ｌｏｃｕｓ， ＱＴＬ）的分析定位，结果表明，２／１／２个ＱＴＬｓ的亲本ＪＸ１７等位基因分别控制着最大根长、根干重和根茎干重比的表达，对表型变异的解释率分别为１６．４％、１７．０％、１６．４％、１０．４％和 １９．９％；２／１个ＱＴＬｓ的亲本ＺＹＱ８等位基因分别控制着最大根长和根茎干重比的增加，对表型变异的解释率分别为１９．６％、１３．０％和１３．２％。检测到的８个ＱＴＬｓ分别位于水稻的染色体２、３、４、５、６、９和?
Drought resistance is one of the most important traits in rice abiotic study. The report here analyzed several major root traits affecting drought resistance expression with the double haploid population (DH) from ZYQ8 (indica) / JXl7 (japonica) containing 127 lines. After 10 days solution culturing, three rice root traits, Maximum Root Length (MRL), Dry Root Weight (DRW) and Root/ Shoot Ratio of Dry Weight (RSR), were revealed existing significant difference among the DH lines. Using the constructed molecular linkage map from this segregating population, the QTL mapping was conducted among these three root parameters. MRL, DRW, and RSR were found being controlled by 2/ 1 /2 QTLs respectively in JX17, 2/0 /1 QTLs respectively in ZYQ8. Phenotype variance could be explained by 16.4% and 17.0% for MRL, 16.4% for DRW, 10.4% and 19.9% for RSR in JX17, 19.6% and 13.0% for MRL, 13.2% for RSR in ZYQ8. All these QTLs identified were distributed on rice chromosome 2, 3, 4, 5, 6, 9 and 10. Comparing with the other mapping results, one QTL for each trait (L169-CT106A for MRL, G45-G1314A for DRW, G62-G144 for RSR)was identical with the results reported previously.
