杂色云芝产漆酶的发酵条件研究

STUDIES ON FERMENTATION CONDITIONS FOR LACCASE PRODUCTION FROM CORIOLUS VERSICOLOR

本文对杂色云芝（Coriolus versicolor）产漆酶的发酵条件作了研究。结果表明摇瓶实验产漆酶（Laccase）的最佳培养基成分为：可溶性淀粉 2g/L, NH4Cl 24mmol/L, 微量元素混合液 7ml/L, pH3.0柠檬酸—Na2HPO4缓冲溶液 0.01mol/L, KH2PO4 1.4×10-2 mol/L, MgSO4·7H2O 2.03×10-3mol/L, CaCl2·2H2O 6.8×10-4 mol/L, VB1 2.97×10-6 mol/L, 吐温80 4.0g/L, 愈创木酚0.01mmol/L, CuSO4 ·5H2O 0.005mmol/L，最佳发酵条件为培养基初始pH3.0, 菌体生长6d，培养基装量为250ml三角瓶中25ml培养液，25℃条件下振荡培养(150r/min)9d。

The optimal fermentation conditions for laccase production by Coriolus versicolor were studied. The composition of fermentation medium was soluble starch 2g/L, NH4Cl 24mmol/L, mixed solution of trace metals 7ml/L, pH3.0 citric acid-Na2HPO4 buffer 0.01mol/L, KH2PO4 1.4×10-2mol/L, MgSO4·7H2O 2.03×10-3mol/L, CaCl2·H2O 6.8×10-4mol/L, VB1 2.97×10-6mol/L, Tween80 4.0g/L, guaiacol 0.01mmol/L and CuSO4·5H2O 0.005mmol/L, and the optimal conditions were pH of medium was 3.0, 25ml medium in 250ml-flask, and incubation with 150 r/min at 25oC for 9 d.