用PAP法对流行性乙型脑炎急性期患者外周血白细胞中的乙脑病毒相关抗原定位检测

Application of PAP Method Detecting on JEV-Associated Antigen in the Peripheral Blood Leucocyte on Patients with Acute Epidmeic B Encephalitis

1991年7—8月在流行性乙型脑炎收治中,用PAP法对急性期患者外周血白细胞进行乙脑病毒相关抗原检测。结果:总检出率为41.2％(24/51),各型检出率:轻型25.0％(2/8)、通普型44.8％(13/29)、重型42.9%(6/14),三型间检出率无差异(p<0.05)。检出病程最早3天,最迟10天。三种白细胞检出率依次为:中性粒细胞、单核细胞、淋巴细胞,可在三种或二种细胞中同时检出,亦有仅见于一种细胞之中。并就检出意义进行了讨论。

The Epidemic B Encephalitis Viral Associated Antigen (JEV-AAg) in the peripheral blood leucoyte were detected with PAP method in the patients with acute Epidemic B Encephalitis during July to August 1991. Results: The total positive rate of JEV-AAg in leucocyte was 41.2%(21/51). The positive rate and the type of the disease were compared; 25% (2/8) in the mild type, 44.8% (13/29) in the ordinary type and 42.9% (6/14) in the severity type, there was on significant difference between the two types. The JEV -AAg can be detected in the neutrocyte. monocyte and lymphocyte, or all three cells, or two cells, or only one. The positive series was 3—10 days after the onset. The detected results were evaluated in signification.