侵染辣椒的烟草蚀纹病毒的鉴定

Identification of Tobacco Etch Virus Infecting Capsicum annuum

1990年在杨陵从表现花叶畸形的辣椒上分离到H90-2分离物。汁液摩擦接种6科17种植物,病毒可侵染2科8种植物,在白肋烟、三生烟上出现坏死蚀纹,在辣椒、心叶烟、曼陀罗上出现花叶畸形,在苋色藜上出现局部枯斑。该分离物的致死温度为50—55℃,稀释限点10^(-3)×10^(-3),体外保毒期3—5天,桃蚜可传毒。病毒粒体为线状,稍弯曲,长720—750nm,宽13nm,与烟草蚀纹病毒的抗血清有明显的阳性反应。根据这些特性,经初步鉴定该病毒属于马铃薯Y病毒组的烟草蚀纹病毒(TEV)。

An isolate of virus was obtained from Capsicum annuum in Yangling of Shaanxi Province. Virus infected 8 plant species from families out of 17 plant species from 6 families tested. Virus Caused systematic lesion etch on Nicotiana tobacum white Burley and N. tobacum samsun, and mosaic and distorsion on Datura stramonium and Capsicum annuum, and local lesions on Chenopodium amaranticolor, Its thermal inactivation point was 50—55℃ dilution end point was 1000—3000, and longevity in vitro was 3—5 days, Myzus persicae was demonstrated as aphid victor. Virus particles were filamentous, about 720—750 nm x 13 nm. The virus reacted positively with the antiserum of Tobacco etch virus by immunodif fusion tests.On the basis of these characteristics, the virus isolate was considered to be identical to TEV of potyvirus group.