四种血清学方法检测烟草TMV、CMV的比较

Comparison of Four Serological Methods for Detecting CMV and TMV in the Crude Extracts of Diseased Tobacco Leaves

本文应用ELISA-异种动物抗体双夹心法(DSM-ELISA)、ELISA-A蛋白酶联法(SPA-ELISA)、ELISA-斑点免疫法(Dot-ELISA)和葡萄球菌凝集法(SA-test)等四种血清学方法检测TMV、CMV感染的烟草病叶,结果以Dot-ELISA法灵敏度最高,其检测病叶粗汁液的最大稀释度为1:1280—2560,其次是DSM-ELISA和SPA-ELISA,均为1:640,SA-test灵敏度较低,为1:80。SPA-ELISA的非特异性反应比DSM-ELISA低,用健康汁液吸附抗血清,可以明显降低Dot-ELISA和SA-test的非特异性反应。

Three ELISA and an SA-test methods were used to detect CMV and TMV in the crude extracts of diseased plant. For the sensitivity, Dot-ELISA method is the best with maximum dilution point of 1.28—2.56×10^(-3), followed by DSM-ELISA and SPA-ELISA (6.4×10^(-2)), SA-test is lower(0.8× 10^(-2)). For the nonspecific reaction, SPA-ELISA is lower than DSM-ELISA. When the antisera are absorbed by the extracts from the health plants, it was avoided to a great extent in Dot-ELISA and SA-test.