杆状病毒杀虫剂安全性评价的历史和现状

Estimate the Safety of Baculovirus Insecticides:the History and the Status Quo

从 70年代以来学者们用各种杆状病毒做了大量的针对各类生物甚至人类的安全性试验 ,几乎所有试验都证明杆状病毒是安全的 ,但也有个别试验得到不同结论 ,在这些试验的论文发表后曾引起学术界两次大的争论 ,这些不同结果后来均被其他学者甚至作者本人用实验否定。 90年代以来杆状病毒大量作为载体表达外源基因 ,其中有些论文报道了在哺乳动物细胞中的表达 ,引起对杆状病毒安全性的怀疑 ,本文对此进行了分析总结。本文还利用自己研制的重组杆状病毒杀虫剂的安全性试验对鲜有报道的重组杆状病毒杀虫剂安全性进行了论述。作者认为杆状病毒杀虫剂包括重组杆状病毒杀虫剂都是安全。

This article reviews the evaluation of security of baculovirus used as a pesticide.Since 1970s,the scholars have done a lot of experiments with kinds of baculovirus to test the security of a large number kinds of living things even our human beings.Almost all experiments proved that baculovirus is secure,but some experiments came to different conclusions.These gave rise to great debate twice when they were published,but these conclusions have been proved to be wrong with later test by other scientists or the author himelf.Since 90s baculovirus have been used a great deal as the vector to express the foreign gene.Some of them reported the expression in mammalian cells,which brought the suspicious of the baculovirus safety.This article made an analysis and a conclusion about them.Also,this article laid emphasis on the security of recombination baculovirus with the results of the security experiment of self\|made recombination baculovirus pesticide.In the last analysis,this article draws a conclusion that the baculovirus and recombinant baculovirus insecticides are secure.