全基因组基因表达频谱研究的新方法——SAGE和IPGI

New Techniques for Genome-Wide Analysis:SAGE and IPGI

SAGE和IPGI是新近发展起来的用于全基因组基因表达频谱分析和寻找差异基因的新技术 ,可以同时反映正常或异常等不同功能状态下细胞整个基因组基因表达的全貌 ,特别是对低丰度表达基因有较高的检测结果 ,因而具有重要的应用价值。本文简介SAGE和IPGI技术的基本原理、操作方法及其应用前景。

Analysis of gene expression can provide information to understand changes of cells in normal or pathological situations.Traditional methods for gene analysis are limited.Here we described two newly developed techniques which can be used to the analysis of the whole genome genes,especially the low expression genes.