接种Glomus versiforme对红三叶草利用有机磷的影响

EFFECT OF INOCULATED GLOMUS VERSIFORME ON UTILIZATION OF ORGANIC PHOSPHORUS BY RED CLOVER

以红三叶草为材料 ,利用三室隔网培养方法 ,研究接种菌根真菌Glmousversiforme对土壤有机磷及外加有机磷化合物植酸钠 (Na Phytate)、核糖核酸 (RNA)和卵磷脂 (Lecithin)的利用效率 .植株生长 7wk后收获测定植株干物重、含磷量和根系菌根侵染率 .结果表明 :接种菌根真菌能明显增加植株干物重、含磷量和吸磷总量 .与各有机磷处理相比 ,无机磷 (KH2 PO4 )处理生长效应最好 .施用有机磷化合物各处理与CK相比均明显促进了植株生长 ,但不同有机磷处理之间没有显著差异 .在植株吸磷量上 ,各接种处理均为相应CK处理的 2倍以上 ,其中磷酸二氢钾处理吸磷量最高 .菌根对照植物的根外菌丝虽然被限制在有限的空间内 ,但其吸磷量也达到不施磷的CK -M处理的 2倍多 .这说明由于菌根的形成植物能利用土壤固有的有机磷来满足自身生长的需要 .表 3参

In order to study the role of VAMF(Vesicule Arbuscular Mycorrhizal Fungus) hyphae in absorbing phosphorus from organic phosphorus sources, a experiment was carried out in pots under controlled condition. There were three compartments in the test pots,and the root compartment was separated from the hyphal compartment by a nylon net with 30 μm mesh. In the mycorrhizal control, the root compartment was separated from the hyphal compartment by 0.45 μm membrane. Red clover was grown in the root compartments inoculated or uninoculated with VAM fungus Glomus versiforme. Different organic phosphorus sources were added uniformly in the hyphal compartments except the control treatments, and no phosphorus was applied. The results showed that mycorrizal infection significantly increased phosphorus uptake of red clover from different phosphorus sources and greatly promoted the growth of plants. Inorganic phosphorus(KH 2PO 4) had much better effect on growth and P uptake of plants than organic phosphorus, but no significant differences were found from all inoculated treatments. The results clearly demonstrated the efficiency of VAM hyphal in utilization of organic phosphorus by red clover. Plants inoculated with Glomus versiforme were effective in hydrolysis of organic phosphorus and confirmed the important direct contribution of VAM hyphae to plant P nutrition. Tab 3, Ref 10