载脂蛋白E基因多态性与阿尔茨默病的关系探讨

Study on the Relationship between Apolipoprotein E Gene Polymorphism and the Alzheimer Desease

目的 :探讨阿尔茨默病 (AD)患者的载脂蛋白E(ApoE)基因的分布 ,分析它们的相互关系。方法 :利用聚合酶链式反应 -限制性片段长度多态性 (PCR RFLP)技术对AD患者和相应健康对照的ApoE基因进行分型 ,从而进行AD与ApoE等位基因多态性的关联分析。结果 :AD病人ApoE等位基因ε4 占 2 0 .74% ,明显高于正常人对照组的 7.6 4% ,而ε3 则占 6 4.6 3% ,低于正常人对照组的 84.12 % ,均有极显著性差异 (P <0 .0 1)。结论 :ApoE等位基因中 ,ε4 可能为AD的易感因子 ,而ε3 则为保护因子。

Objective: To study the distribution of ApoE gene in patients with Alzheimer desease (AD) and the relationship between them. Method: ApoE genotypes were determined by PCR RFLP technique,and the correlation between ApoE allele polymorphism and AD was analyzed. Results: ApoE ε 4 allele in patients with AD was 20.74%,significantly higher than that in the control group(7.64%), and ε 3 allele in patients with AD was 64.63%,significantly lower than that in the control group (84.12%). Conclusion: The ApoE allele ε 4 may be a genetic susceptible factor to AD,and ε 3 allele be a protection factor.