迷走神经切断对胃肠道给予大鼠伤寒杆菌诱发的下丘脑室旁核和视上核Fos表达的影响

The influence of vagotomy on Fos expression in PVN and SON induced by Salmonella Typhimurine infection of gastrointestinal tract in the rat

为研究迷走神经在自然感染状态下向脑传递免疫信息的作用 ,应用免疫组织化学方法 ,观察了切断膈下迷走神经对大鼠消化道内给予鼠伤寒杆菌刺激诱发的下丘脑室旁核和视上核的Fos表达变化的影响。结果发现 ,接受细菌刺激的动物与仅给予生理盐水的动物相比 ,回肠和肠系膜淋巴结有明显炎症存在 ,室旁核外侧部和视上核背侧部的Fos阳性细胞数增加 ;膈下迷走神经切断后 ,手术 +细菌组与假手术 +细菌组相比 ,室旁核的外侧部和视上核背部Fos表达减少。因此迷走神经途径在自然感染性免疫应答过程中 ,特别是在其早期阶段可能是传递腹腔免疫信息的重要途径之一。

To study the role of vagus nerve in conveying immune information into the brain under the condition of natural infection, orally induced infection of Salmonella Typhimurine (STM) was used to mimic the condition of natural antigens challenge, changes of Fos expression in paraventricular nucleus of hypothalamus (PVN) and supraoptic nucleus (SON) induced by STM following vagotomy were observed with immunohistochemical staining technique. Results showed that in bacteria-stimulated rats, inflammation existed in intestinum and mesenteric lymphonodi, Fos-LI cells increased in lateral part of PVN and dorsal part of SON. Subdiaphragmatic vagotomy attenuated Fos-LI cells in lateral part of PVN and dorsal part of SON in bacteria-stimulated rats. It is suggested that vagus nerve probably is one of the important pathways transmitting abdominal immune information into the brain, especially in the early phase of immune response.