瘦素在胎盘组织中的表达及其与新生儿体重的关系

Study on the relationship between leptin and neonatal weight and the expression of leptin in placenta

目的 探讨瘦素在胎盘组织中的表达及其与新生儿体重的关系。方法 采用放射免疫法 (RIA)检测 10 0例足月孕妇静脉血及其新生儿脐血瘦素水平 ,根据新生儿出生体重分为大于胎龄儿(LGA)组 19例 ,适于胎龄儿 (AGA)组 6 5例 ,小于胎龄儿 (SGA)组 16例 ,同时采用逆转录 聚合酶链反应(RT PCR)技术 ,检测 41例胎盘组织中瘦素mRNA表达水平。结果 (1)胎盘组织中瘦素mRNA表达水平为 0 97± 0 0 4,与新生儿体重呈显著正相关关系 (r =0 43,P <0 0 1)。其中LGA组 (1 0 1± 0 0 3)显著高于AGA组 (0 97± 0 0 2 ) ,SGA组 (0 93± 0 0 3)显著低于AGA组 ,差异均有极显著性 (P <0 0 1)。(2 )母血瘦素水平为 (14 2 2± 7 6 6 ) μg/L ,与新生儿体重无相关关系 (r=0 11,P >0 0 5 )。 (3)新生儿脐血瘦素水平为 (7 5 8± 5 15 ) μg/L ,与新生儿体重呈显著正相关关系 (r=0 5 7,P <0 0 1) ,其中LGA组脐血瘦素水平为 (13 38± 6 75 ) μg/L ,显著高于AGA组的 (7 40± 4 45 ) μg/L ,SGA组为(2 79± 1 5 4) μg/L ,显著低于AGA组 ,差异有极显著性 (P <0 0 1)。结论 脐血及胎盘组织中瘦素水平与胎儿生长发育状态密切相关 ;母血瘦素水平与新生儿体重无关 ;

Objective To investigate the relationship between the leptin level and neonatal weight and the expression of leptin in placenta. Methods The concentrations of leptin in 100 maternal blood and umbilical blood of the term pregnant women were examined by radioimmunoassay (RIA). According to the neonatal weight to divide into the large for gestational age (LGA) group 19 cases, the appropriate for gestational age (AGA) group 65 cases, the small for gestational age (SGA) 16 cases. The level of leptin mRNA in 41 placental tissue was examined by Reverse transcription polymerase chain reaction (RT PCR). Results (1) The expression level of leptin mRNA in placenta was 0 97±0 04, which was positively related to the neonatal weight significantly ( r =0 43, P <0 01). The expression level of the LGA group (1 01±0 03) was significantly higher than the AGA group (0 97±0 02), ( P <0 01). The expression level of the SGA group (0 93±0 03) was significantly lower than AGA group, ( P <0 01). (2)The concentration of leptin in maternal blood was (14 22±7 66) μg/L, which was not related to the neonatal weight ( r =0 11, P >0 05).(3)The concentration of leptin in umbilical blood was (7 58± 5 15) μg/L, which was positively related to the neonatal weight ( r =0 57, P <0 01). The concentration of leptin of LGA group was significantly higher than that of AGA group, which was (7 40±4 45) μg/L ( P <0 01). The concentration of leptin of SGA group was (2 79±1 54) μg/L, significantly lower than that of AGA group,( P <0 01). Conclusions The level of leptin in umbilical blood and placenta is closely related to the growth state of fetus. The level of leptin in maternal blood is not related to the neonatal weight. The placenta of pregnant woman is an important resource of leptin.