黄芪对辐照小鼠粒-单系造血祖细胞的影响

Effects of Radix Astragali on CFU-GM in irradiated mice

目的 :探讨黄芪对造血功能的影响及作用机制。方法 :采用造血祖细胞体外培养和体内扩散盒植入技术 ,观察黄芪对辐照小鼠粒 单系造血祖细胞 (CFU GM)的影响。结果 :体内培养证明 ,辐照前、后用药组CFU GM分别为 (2 7 14± 5 88)个 / (4× 10 5BMC)和 (2 6 38± 4 90 )个 / (4× 10 5BMC) ,均较辐照对照组 (16 5 8± 3 37)个 / (4×10 5BMC)明显增高 (P <0 0 1) ,而体外培养未显示刺激活性。结论 :黄芪可刺激体内造血干细胞的增殖与分化 ,对辐照小鼠的骨髓具有保护作用。

Aim:To study the effects of Radix Astragali on hemopoiesis and its mechanism.Methods:By using cell culture of hemopoietic progenitors in vitro and diffusion chamber implantation in vivo,we observed the effects of Radix Astrogali on colony forming unit granulocyte /macrophage(CFU GM).Results:In diffusion chambers in vivo,the number of CFU GM were(27 14±5 88)/(4×10 5BMC) and(26 38±4 90)/(4×10 5BMC) in the Radix Astragali pretreated or treated groups,respectively,which were significantly higher than that of irradiated control group( P <0 01),but there was no stimulation activity in vitro on irradiated mice.Conclusion:Radix Astragali can stimulate the proliferation and differentiation of the CFU GM in diffusion chambers in vivo and may protect bone marrow of irrdiated mice.