NGF诱导PC12细胞分化的神经细胞行为观察

Observation on differentiated neuron behaviour induced by NGF in cultured PC12 cells

目的 :观察PC12细胞分化的神经细胞的分裂、突起延伸、迁徙和分化等细胞行为。方法 :采用Giemsa、甲绿 派郎宁染色和增殖细胞核抗原 (PCNA)免疫组织化学染色方法观察培养的PC12细胞。结果 :培养分化的神经细胞呈现横裂、纵裂等不同方式的无丝分裂像 ;分裂中的神经细胞可呈现形态及化学不对称性 ;分化的神经细胞有明显的细胞迁徙运动和突起延伸。结论 :NGF诱导PC12细胞分化的神经细胞能以无丝分裂方式增殖 ;分化神经元可以通过细胞迁徙、突起延伸及共同胞质桥等方式相连接。

Aim:To study the cell division, neurite extension, migration of neurons differentiated by NGF in subcultured PC12 cells.Methods: The Giemsa staining, methyl green pyronin staining and proliferating cell nuclear antigen immunohistochemical assay were used to study the subcultured PC12 cells.Results: The differentiated neuron appeared different patterns of amitotic figures such as transverse sever, longitudinal split, etc The proliferating neurons divided in an asymmetric way morphologically and chemically. The differentiated neuron appeared an obvious movement of cell migration and neurite extension.Conclusion: The neurons differentiated by NGF in cultured PC12 cells can divide in amitotic way.The differentiated neuron can form junction by cell migration, neurite extension or cytoplasmic bridge.