转化生长因子β_1对口腔鳞癌细胞系血管内皮生长因子的影响

Transforming growth factor beta 1 differently modulate activity of vascular endothelial growth factor In oral squamous cell carcinoma and metastasis carcinoma cell lines

目的 探讨转化生长因子 β1(TGF β1)和口腔鳞癌侵袭转移的关系。方法 采用ELISA和免疫组织化学法测量不同浓度TGF β1作用于口腔鳞癌TSCCa细胞系及颈淋巴转移癌GNM细胞系后血管内皮生长因子(VEGF)活性和阳性表达的变化。结果 不同浓度的TGF β1作用于TSCCa 12h后 ,VEGF活性和阳性表达与对照组相比 ,无明显差异 (P >0 .0 5 ) ;而不同浓度的TGF β1作用于GNM细胞 12h后 ,VEGF活性和阳性表达与对照组相比 ,有显著性差异 (P <0 .0 5～ 0 .0 1) ,VEGF活性和阳性表达与TGF β1有剂量依赖关系。结论 TGF β1可能通过调节VEGF的活性和阳性表达的方式来参与口腔鳞癌侵袭转移。

Objective To detect the role of transforming growth factor β_ 1(TGF-β_ 1) on the invasiveness and metastasis of human oral squamous cell carcinoma .Methods Using ELISA and immunhistiochemistry detect the activity and expression of VEGF in oral squamous cell carcinoma cell lines TSCCa and metastasis carcinoma cell lines GNM treated by different levels of TGF-β_ 1. Results TGF-β_ 1 had no effect on the activity and postive expression of VEGF in TSCCa cell lines. While TGF-β_ 1 had increased the activity and postive expression of VEGF in GNM cell lines, Moreover, the activity and postive expression of VEGF had more dependent on TGF-β_ 1. Conclusion TGF-β_ 1 may be a factor associated with invasive and metastasis potential of oral squamous cell carcinoma.