口腔癌前病变患者博莱霉素敏感性反应与宿主细胞再活化反应的相关研究

The coreelation research on bleomycin induced sensibility and host cell reactivation in the patients with oral precancerous lesion

目的 了解口腔癌前病变患者的细胞再活化反应 (HCR )和博莱霉素敏感性反应 (BIS)的变化 ,以及与病变的相关性。方法 选择 16例口腔癌前病变患者 (白斑、赤斑、慢性盘状红斑狼疮、口腔扁平苔藓 ,活检证实均有上皮异常增生 ) ,16例正常对照。两组年龄、性别分布相似 ,均进行HCR、BIS检测和吸咽、饮酒、肿瘤家族史的调查。HCR检测反应被检细胞的DNA修复功能。BIS检测是用博莱霉素作突变剂 ,反应细胞对致突变剂的敏感性。统计学方法采用t检验、相关分析和多元回归分析。结果 癌前组HCR值低于对照组 (P <0 .0 5 ) ;HCR与BIS相关系数为 -0 .5 60 (P <0 .0 0 1) ,烟酒相关系数为 0 .5 12 (P <0 .0 1) ;HCR为一项有意义的独立危险因素存在(OR∶3 .2 5 ,95 %CI =1.0 0 74～ 10 .4889)。结论 癌前病变组存在核酸切除修复功能的下降。HCR较BIS为一项更敏感的指标 ,可能成为癌前病变易感性指标。

Objective To investigate the changes of bleomycin induced sensibiligy(BIS) and host cell reactivation in the patients with precancerous lesion,to analyze the correlation of the two indicators and precancerous lesion.Methods The study was conducted among 16 normal controls and 16 cases of precancerous lesion(including leukoplakia,erythroplakia,discoid lupus erythema and oral lichen planus.All the cases had abnormal epithelia hyperplasia proved by biopsy).The two groups were similar in age and sex.All the subjects were examined by HCR and BIS,and in the meantime,the dangerous factors of smoking,alcoholic drinking and family tumor history were also investigated.HCR reflects DNA repair capacity of lymphocyte (especially,the cutting repair function).However,BIS was to take bleomycin as a mutagen to reflect the sensibility of lymphocyte to the mutagen.t-test,analysis of correlation and multivariate regression were adopted in statistics.Results The values of HCR in the precancerous group were lower than that of the control,The correlation coefficient of HCR and BIS was -0.560(P<0.01),and that of smoking and alcohol was 0.512(P<0.01),HCR acts as an independent risk factor in the occurrence of oral precancerous lesion(OR 3.25,95%CI=1.0074～10.4889).Conclusion There is a fall in the function of nucleic acid excision in the precancerous group.HCR is more sensitive than BIS,likely predicting genetic sensitivity for precancerous lesion.