免疫PCR方法在血清抗Sm抗体测定中的应用

Detection of anti-Sm antibodies in SLE serum by immuno-PCR

目的 :将免疫PCR方法应用于血清抗Sm抗体测定中。方法 :采用SaHIgG DNA探针建立了血清抗Sm抗体免疫PCR检测方法。结果 :该法测定SLE患者血清抗Sm抗体的特异性强 ,敏感性是ELISA方法的 10 7倍。批内和批间变异系数分别为 2 3%～ 4 8%和 3 7%～ 6 9% ,而且与ELISA方法呈高度正相关。结论

Objective:To apply immuno-PCR for detection of anti-Sm antibodies.Methods:Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe The experimental conditions were optomized and methological identification indicated obvious increase(×10 7 ) in sensitivity compared with that of ELISA The positive rate of anti-Sm antibodies was 54 9% in SLE sera,and significantly higher than that of ELISA Intra- and inter-CV were 2 3%～4 8% and 3 7%～6 9% respectively Immuno-PCR was positively correlated with ELISA(P<0 001) Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically