白细胞介素6(IL-6)和IL-8mRNA在变应原诱导的鼻粘膜晚期反应标本的表达

Messenger RNA expression of IL-6 and IL-8 in allergen-induced late-phase cutaneous reactions (LPR) in Schneiderian membrane subjects

目的 :测定IL 6和IL 8变应原诱导的鼻粘膜晚期反应标本中mRNA的表达。方法 :采用原位杂交技术 ,测定变应原诱导的 10例变态反应性鼻炎病人的鼻粘膜标本表达IL 6和IL 8mRNA阳性细胞数。结果 :在 10例标本中 ,2种细胞因子的阳性表达率分别为 9/ 10和 10 / 10。与对照相比 ,变应原诱导的鼻粘膜标本表达IL 6和IL 8mRNA阳性细胞数明显增加 (P<0 0 5和P <0 0 1)。结论 :IL 6和IL

Objective:To detect mRNA expression of IL-6 and IL-8 in allergen-induced late-phase cutaneous reactions in schneiderian membrane subjects Methods:Cryostat sections from rhinitis biopsies from 24 h allergen-induced late-phase cutaneous reactions (LPR) in 10 human atopic subjects were hybridization with 35 S-labeled RNA probes for IL-6 and IL-8 Results:mRNA was detected for IL-6 (9/10) and IL-8 (10/10) Compared with the control, there were significant increases in the numbers of cell expressing mRNA expression for IL-6 and IL-8 (P<0 05,P<0 01) Conclusion:The augmentation of mRNA expression of IL-6 and IL-8 maybe regarded as the mark of rhinitis in LPR