原位分子杂交检测尖锐湿疣组织中HPV　DNA的研究

Detection of HPV DNA in patients with condyloma acuminatum using hybridization in situ

本文采用ＨＰＶＤＮＡ原位分子杂交法检测了３０例尖锐湿疣组织标本，结果显示ＨＰＶ６／１１型阳性检出率为８６．６％，ＨＰＶ１６／１８型为１６．６％，ＨＰＶ３１／３３／３５则为阴性，总的阳性检出率为９０％。阳性结果除见于表皮浅层外，还见于棘细胞层中下部及基底细胞层内。作者认为原位分子杂交法是目前检测ＨＰＶ感染及其分型的一种敏感、特异、快速且相对简便的方法，又能进行感染的组织学定位，同时这一方法还适应于对以往病例进行回顾性调查。

Skin specimens from 30 patients with condyloma acuminatum were examined using HPV DNA hybridization in situ.The results showed that the positive rates of HPV 6/11,HPV 16/18 and HPV 31/33/35 were 86. 6% (26/30),16. 6% (5/30) and 0%(0/30) respectively.The total positivity rate was 90%(27/30). The positive reactants were found in the lower and mid-epidermis and basal cells in addition to upper epidermis. The authors consider that hybridization in situ is a sensitive,specific and rapid technique for detecting HPV infection and HPV DNA typing. HPV infection can be histologically localized. Besides,this technique can be used for retrospective studies.