肌腱切断后骨骼肌的退变与再生

The Degeneration and Regeneration of Skeletal Muscle After Tenotomy

以纯系ＢＡＬＢ／Ｃ小白鼠１５只为实验动物，切断一侧腓肠肌肌腱。通过光镜、电镜及横纹肌肌动蛋白（ＭＡ）、肌红蛋白（ＭＧ）免疫组织化学染色等方法，观察切断肌腱后不同时间腓肠肌的形态改变，横纹肌特异蛋白表达的变化。结果发现：切断肌腱后１，２和４周均有肌纤维退变，再生成肌细胞移入退变的肌纤维。肌动蛋白和肌红蛋白的分布发生变化，到４周时肌红蛋白染色肌纤维全部为阳性，失去红白肌纤维型别差异。因此，肌腱切断后一方面肌纤维内部结构紊乱，另一方面存在持续的退变和再生反应，再生的肌细胞与原肌纤维融合。

In order to investigate the degeneration and regeneration of skeletal muscle fiber after tenotomy, the tenotomy were carried out on gastrocnemius muscles in 15 adult BALB/C mice. The tenotomized muscles were removed on 1,2 and 4 weeks postoperatively. Specimens were processed for histological study with light microscope and TEM, and skeletal muscle specific protein expression analysis with myoactin (MA) and myoglobin (MG) immunohistochemical staining. The results showed that the regenerating muscle cells and the degenerating muscle fibre coexisted in tenotomized muscle. The internal structure and histochemical pattern of muscle fiber changed after tenotomy. The regenerating muscle cells fused with degenerating fibres.