龈下优势菌在冠、桥修复材料表面定植量的测定

The Study of Precedent Subgingival Flora Colonization on Four Kinds of Crown and Bridge Restorative Materials

为考察冠、桥修复材料对微生物定植的影响，探讨微观评价冠、桥修复材料的标准，本研究选用ＳＤＡ－Ⅱ型中熔合金、烤瓷熔附金属材料、湿热固化冠用塑料、ｐｌａｔ铸造陶瓷４种材料和血链球菌、粘性放线菌、具核梭杆菌、黄褐二氧化碳噬纤维菌４种龈下优势菌，通过３，７，１４ｄ连续厌氧培养，测定试件ＰＢＳ洗涤液中微生物ＯＤ值，考察定植在材料表面的微生物量。本研究表明，不同细菌对不同材料定植量不同，与材料表面的结构、成分。

The purpose of this in vitro study was to investigate the effects of microbial colonization on different crown or fixed bridgeused restorative materials and discuss the standards for evaluating those materials microscopically. Four kinds of restorative materials such as SDAⅡ type medium alloy, ceramicfusedtometal material, heat cure composite resin for crown restoration, Plat castable ceramics and four species of precedent subgingival flora (A.viscosus, F.nucleterm, C.ochracea, S.sanguis) were used. The material specimen had been continuously anaerobic cultured with the experimental bacterial fluid for 3,7 and 14 days respectivelly, then the OD values of the specimenwashing fluid which could stand for the biomass of colonized bacteria on the surfaces of materials were measured. The results indicated that the quantity of precedent subgingival flora colonization on the surfaces of different restorative materials varied among different species of bacteria, which might concern with the surface structure, composition, property of anticorrosion and antisoluability of those materials.