生长因子网络调节对骨形成作用的研究Ⅱ.IGF-Ⅱ对人胚成骨样细胞增殖及分化的影响

The Effects of IGFⅡ on the Proliferation and Differentiation of Human Osteoblastlike Cells

利用体外分离培养人胚成骨样细胞，加入不同剂量胰岛素样生长因子－Ⅱ（ＩＧＦ－Ⅱ），从时间－效应和剂量－效应两个方面考察ＩＧＦ－Ⅱ对人胚成骨样细胞增殖与分化的影响。研究结果表明，ＩＧＦ－Ⅱ的促骨形成效应显著，它对体外培养的人胚成骨样细胞ＤＮＡ、胶原蛋白和碱性磷酸酶合成均有明显的促进作用，其作用亦呈剂量与效应相依赖的关系，在４个含量组中，１０ｎｇ／ｍｌＩＧＦ－Ⅱ对成骨样细胞的作用最强。ＩＧＦ－Ⅱ是一种对成骨细胞作用较全面的生长因子。

Biological regulatory mechanism which supports bone cell development within the context of proliferation/differentiation relationship serves as a viable basis for addressing skeletal disorders and provides experiment and theoretic basis for selection and ultilization of growth factors to reconstruct bone defect.Osteoblastlike cells isolated from human calvarium were used in this study.IGFⅡ were investigated by adding in the culture medium with different concentration. The results showed that IGFⅡ stimulated DNA,collagen synthesis and ALP activity of human osteoblastlike cells.Its effects were strong for proliferation and differentiation of osteoblast cells, when 10ng/ml of four different concertrations (0.1,1,10 and 100 ng/ml) were used.IGFⅡ was proved to be important factor in bone regeneration.