摘要
用冷乙醇沉淀法从变形链球菌培养上清液或用0.5mol/L磷酸盐缓冲液、6mol/L脲和2%SDS溶液从变形链球菌细胞表面分别粗提变形链球菌表面粘结素。用SDS聚丙烯酰胺凝胶电泳和细菌粘附抑制实验比较4种粘结素粗提物的种类及粘附活性。结果表明4种粘结素粗提物蛋白组成相似,且4种粗提物均有较强的粘附抑制活性,而从培养上清液中提取变形链球菌粘结素对蛋白生物活性损伤小,方法简便易行。
The adhesins of S.mutans serotype c endemic strain WD9463A were extracted from culture superant by cold ethol precipitation, or were extracted from cell surface by 0.5 mol/L phosphate buffer, 6 mol/L urea or 2% SDS solution. The composition and adhesive activities of the extracts were compared by SDSPAGE and bacterial adhesion inhibition experiments. The results showed that the protein composition of the four extracts were similar, and all extracts inhibited the adhesion of the endemic strain dramatically. It indicates that four methods can be used to extract adhesins of S.mutans, but extracting adhesins from culture superant is better than the others.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1998年第1期73-75,T004,共4页
West China Journal of Stomatology
基金
国家自然科学基金