变形链球菌对唾液获得性膜粘附机理的研究Ⅰ.变形链球菌表面粘结素粗提方法的比较

Study on the Adhesion of An Endemic Strain of Streptococcus Mutans Serotype C to Acquired Pellicle Ⅰ. A Comparison of the Methods to Extract Adhesins from Streptococcus Mutans

用冷乙醇沉淀法从变形链球菌培养上清液或用０．５ｍｏｌ／Ｌ磷酸盐缓冲液、６ｍｏｌ／Ｌ脲和２％ＳＤＳ溶液从变形链球菌细胞表面分别粗提变形链球菌表面粘结素。用ＳＤＳ聚丙烯酰胺凝胶电泳和细菌粘附抑制实验比较４种粘结素粗提物的种类及粘附活性。结果表明４种粘结素粗提物蛋白组成相似，且４种粗提物均有较强的粘附抑制活性，而从培养上清液中提取变形链球菌粘结素对蛋白生物活性损伤小，方法简便易行。

The adhesins of S.mutans serotype c endemic strain WD9463A were extracted from culture superant by cold ethol precipitation, or were extracted from cell surface by 0.5 mol/L phosphate buffer, 6 mol/L urea or 2% SDS solution. The composition and adhesive activities of the extracts were compared by SDSPAGE and bacterial adhesion inhibition experiments. The results showed that the protein composition of the four extracts were similar, and all extracts inhibited the adhesion of the endemic strain dramatically. It indicates that four methods can be used to extract adhesins of S.mutans, but extracting adhesins from culture superant is better than the others.