HPLC、GC-ECD测定硝酸甘油血药浓度方法比较

IMPROVED GC-ECD AND HPLC ASSAY FOR THE BLOOD CONCENTRATION OF NITROGLYCERIN

采用高效液相色谱(HPLC)、气相-电子捕获器(GC-ECD)两种不同仪器对硝酸甘油的血药浓度进行测定.结果表明,HPLC最低检测限为血中500ng/ml,日内、日间差异均小于10%,回收率大于70%.GC-ECD最低检测限为血中200pg/ml,日内、日间差异均小于10%,回收率大于70%.证明GC-ECD是可靠、稳定、重复性强的测定方法.可以指导临床医生合理应用GTN制剂,改善冠心病心绞痛患者的生存质量,减少冠心病心脏猝死的发生.

In order to choose an ideal determined measure of blood concentration of nitro-glycerin (glyceryl trinitrate nitroglycerin) in human body,HPLC (high performance liquid chromatography) and GC-ECD (Gas chromatographic-electron capture direction) two different kinds of instruments were used to perform to measure blood concentration of nitroglycerin. By comparing the indexes such as sensitivity,precision,rate of recovery and evaluating the above measure,we choose a stable and reliable GC-ECD measure which was requested by human pharmacokinetics. The study results showed that the lowest sensitivity in plasma was 500ng/ml by HPLC,the inter-run assay precision and accuracy were within 10%,the recovery was greater than 70%,the method was stable,but the sensitivity wasn't satisfied with the clinical need. The sensitivity in plasma was 200pg/ml by GC-ECD, the inter-run assay precision and accuracy were within 10% ,the recovery was greater than 70%,it's a stable,reliable and repeating method. In this study we made Chinese nitroglycerin pharmacokinetics models to instruct the clinical doctor to use nitroglycerin betterly to improve patients life quality to decrease sudden death.