新生小鼠肝细胞的体外培养

ISOLATION AND LONG-TERM CULTURE OF NEW-BORN MOUSE HEPATOCYTES IN VITRO

探讨体外培养新生小鼠肝细胞的方法。方法 :采用肝组织块外植培养和肝组织块胶原酶消化法培养新生小鼠肝细胞 ,并比较两种方法。结果 :运用这两种方法成功地培养出原代肝细胞 ,并能够传代 ,光镜证实培养细胞为肝细胞。结论

Objective:Culture methods of new-born mouse hepatocytes were studied and discussed.Methods:The methods of planting culture of hepatic tissues and collagenase digestion of hepatic tissues were used.And differences between two methods were discussed.Results:Primary culture of new-born mouse hepatocytes has been established by the use of two methods.Mouse hepatocytes have reached 4 population doublings.Hepatocytes were confirmed by light microscope.Conclusions:The ues of these two methods for mouse hepatocyes culture proved to be successful.These techniques lay foundation for the use of hepatocytes for hepatology.